Journal of Molecular Genetics and Gene Research
Population Variation and Polymorphism of the Cotton Pink Bollworm Pectinophora Gossypiella (Saunders) (Lepidoptera: Gelechiidae) Using Inter Simple Sequence Repeats (ISSRS) as Molecular Markers
Abstract
Hanan Salah El Din Taha
Background The most dangerous pest infesting Egyptian cotton plants is the pink bollworm Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae). Substantial insecticide applications required for effective control in production season, resulting insecticide resistance problems. Some investigation ideas were floating for sponsored management. Thus, PCR based examination of pest population DNA genetic diversity using eight Inter-Simple Sequence Repeats (ISSRs) primers screening for population differentiation based on similarity in the pattern of SDS-page bands for larvae.
Results We detected 117 loci with an average population polymorphism of 97.64%. Genetic distance and similarity between test groups were calculated and UPMGA phylogenetic tree were constructed. The average primer efficiency calculated value of PIC and other component were detected for Sharkia, Benisuef and Fayoum governorate. Number of identified alleles Na and the effective number of alleles Ne was attained. Principal component, correspondence analysis and AMOVA results revealed great variance between populations, in addition to moderate level of gene flow, and Infinite diversity distinguished by Fixation index Fst across the genotypes. Estimated Shannon’s index (I) parameter as Nei’s genetic diversity, eveness, estimated diversity, and species richness all results indicates a high level of genetic variation exists among the three geographical sample populations of the same pest.
Conclusions All results statistics indicates a distinct level of genetic variation exists among the three geographical samples of the P.gossypiella field collections. And the PCR technology appear to be effective molecular marker for this investigations.